AT‐rich interaction domain 5A regulates the transcription of interleukin‐6 gene in prostate cancer cells

Interleukin‐6 (IL‐6) is a pleiotropic cytokine that confers androgen‐independence and aggressiveness in prostate cancer (PCa); however, the molecular mechanisms regulating IL‐6 expression remain unclear. The expression of ARID5A, an AT‐rich interaction domain (ARID) DNA‐binding motif‐containing transcription factor is positively correlated with IL‐6 expression in human PCa. We, therefore, hypothesized that ARID5A could regulate IL‐6 expression in PCa. The relationship between ARID5A and IL‐6 in PCa patients was analyzed using statistical analyses of multiple clinical microarray data sets. To investigate whether ARID5A regulates IL‐6 expression, CRISPR‐driven ARID5A knockout clones were established in DU145 and PC‐3 cells...