Functional genomics of the human epididymis: Further characterization of efferent ducts and model systems by single cell RNA‐seq analysis

This study used single-cell RNA‐seq technologies to elucidate the identity of cells within the human efferent ducts and compared them to caput epididymis cells. We also compared the cellularity of primary tissues with those of 2D and 3D (organoid) culture models used for functional studies. The data suggest that efferent ducts are lined with a transitional epithelium, which, like the urothelium, can stretch and contract depending on luminal volume. This is consistent with its primary role in seminal fluid resorption and sperm concentration. Single-cell RNA‐seq data from the human epididymis contribute to our understanding of this highly specialized organ.