Single‐cell protein profiling defines cell populations associated with triple‐negative breast cancer aggressiveness

Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype that lacks targeted therapy. In addition, TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single‐cell techniques and next‐generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. The study further classified the epithelial‐mesenchymal plasticity of tumor cells and molecularly defined phenotypically diverse populations of the tumor‐associated stroma. Furthermore, in a retrospective tissue‐microarray TNBC cohort, we show that the level of CD97 at the time of surgery has predictive potential.